AP111

Storage: at -20°C for two years

Description

EasyTaq® DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The
enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE
has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with
unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.

 

Highlights

• Extension rate is about 1-2 kb/min.
• Unique buffer system compatible with PAGE.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T
vectors.
• Amplification of genomic DNA fragment up to 3 kb.

 

Applications

Short fragment PCR

 

Unit Definition

One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

EasyTaq® DNA Polymerase for PAGE has passed the following quality control assays: functional absence of double- and
single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of EasyTaq® DNA Polymerase for
PAGE has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

 

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

 

10×EasyTaq® Buffer for PAGE (with Mg2+)

200 mM Tris-HCl (pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others

 

 

Contents& storage

 

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EasyTaq® DNA Polymerase

  • Views: 802
  • Product Code: AP111
  • Availability: In Stock
  • $55.00

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