Isopropylthio-β-galactoside (IPTG) is an effective inducer of β-galactosidase activity. It is commonly used with X-gal to detect lac gene activity in cloning based on blue/white selection. It is also used as an inducer of protein expression in lac or tac promoter -regulated expression vectors.
• IPTG used with X-gal in blue/white screening.
• Used as an inducer of protein expression under the control of lac or tac promoter