EasyPure® miRNA Kit provides a simple and fast column based method to isolate small RNA (≤200 nt) from cells, tissues, fresh blood and virus. Samples are lysed with lysis buffer. The addition of chloroform to the sample separates the solution into an upper colorless aqueous phase containing RNA, an interphase and a lower organic phase. High molecular RNA (28S rRNA, 18S rRNA, mRNA) is bound to a silica membrane. Small RNA in the flow-through can be bound to a miRNA spin column.
miRNA related research
|Fresh Blood||50-200 μl|