EasyPure® HiPure Plasmid MaxiPrep Kit uses a modified alkaline lysis method to isolate high quality plasmid DNA from ≤ 500 ml (LB) or ≤100 ml (ArtMedia® Plasmid Culture) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit error-free visual identification of complete bacterial cell lysis and neutralization. Endotoxin is removed by a simple incubation on column with a novel buffer. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.
• Fast: the whole procedure can be performed in one hour.
• Simple: endotoxin is removed on column.
• High yield: DNA yield up to 1 mg.
• Error-free visualization: colored buffers to visualize lysis and neutralization.
Restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection