BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).
F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR,TetR)
• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• KanR and TetR.
• Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.