Description

BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype

F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR,TetR)

Features

• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• KanR and TetR.
• Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.

 

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TransB(DE3) Chemically Comptent Cell

  • Views: 157
  • Product Code: CD811
  • Availability: In Stock
  • $130.00

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