Transetta(DE3) Chemically Competent Cell is specifically designed from imported strain for chemical transformation of DNA. It is resistant to chloramphenicol(CamR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).
F- ompT hsdSB(rB- mB-) gal dcm lacY1(DE3)pRARE(argU, argW, ileX, glyT, leuW, proL)(Camr)
• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• tRNAs for 6 rare codons AUA, AGG, AGA, CUA, CCC, GGA. Enhance the expression level of proteins in the prokaryotic
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.