AT321

Description

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The SuperMix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA are suitable for regular PCR, not for qPCR.

 

Highlights

• One-tube format for simple and fast setup and reduce pipetting variability.
• The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA.
• PCR-ready cDNA in 30 minutes (unsuitable for qPCR).
• cDNA up to 12 kb.

 

Applications

Multiple copy and low copy gene detection

 

 

Contents& storage

 

 

Citations & references

 

LiteratureJournalIFAuthorDateLink
Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress Front Plant Sci. 3.948Hongyan Wang, et al. 2015 Sephttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4586422/
Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na+/H+ Antiporter Gene from the Halophyte Kosteletzkya virginica The Scientific World Journal  Hongyan Wang, et al.  2014 Junhttp://www.hindawi.com/journals/tswj/2014/141675/abs/

 

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TransScript®  All-in-One First-Strand cDNA Synthesis SuperMix for PCR

  • Views: 276
  • Product Code: AT321
  • Availability: In Stock
  • $520.00

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