AT311

Description

Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85oC for 5 seconds.

 

Highlights

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 12 kb.

 

Applications

Multiple copy and low copy gene detection

 

 

Contents& storage

 

 

Citations & references

 

LiteratureJournalIFAuthorDateLink
IFN-α as an Adjuvant for Adenovirus-Vectored FMDV Subunit Vaccine through Improving the Generation of T Follicular Helper Cells PLoS One 3.73Su C, et al. 2013 Jun http://www.ncbi.nlm.nih.gov/pubmed/23823532
Natural variation in PTB1 regulates rice seed setting rate by controlling pollen tube growth Nature communication 10.02Shuangcheng Li,  Wenbo Li,et al. 2013 Novhttp://www.nature.com/ncomms/2013/131118/ncomms3793/full/ncomms3793.html
?WT.ec_id=NCOMMS-20131120
Modulation of HBV replication by microRNA-15b through targeting hepatocyte nuclear factor 1α Nucleic Acids Research 8.28Xiaopeng Dai, et al. 2014 Aprhttp://nar.oxfordjournals.org/content/early/2014/04/04/nar.gku260.short
Regulation of Tissue LC-PUFA Contents, D6 Fatty Acyl Desaturase (FADS2) Gene Expression and the
Methylation of the Putative FADS2 Gene Promoter by Different Dietary Fatty Acid Profiles in Japanese Seabass (Lateolabrax japonicus)
PLOS ONE 3.23Houguo Xu, Xiaojing Dong,et al. 2014 Janhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909213/
Cytokinin pathway mediates APETALA1function in the establishment of determinate floral meristem in Arabidopsis.  PNAS 9.737Yingying Han, et al.     2014 Mayhttp://www.pnas.org/content/111/18/6840.short
Process of aerenchyma formation and reactive oxygen species induced by waterlogging in wheat seminal roots Planta 3.347Xu Q T, et al. 2013 Novhttp://www.ncbi.nlm.nih.gov/pubmed/23975011
Natural variation in PTB1 regulates rice seed setting
rate by controlling pollen tube growth
Nature communication 10.02Shuangcheng Li, et al.  2013 Novhttp://www.nature.com/ncomms/2013/131118/ncomms3793/full/ncomms3793.html
?WT.ec_id=NCOMMS-20131120
Chrysin promotes osteogenic differentiation via ERK/MAPK activation Protein and Cell 3.22Zeng W, et al.  2013 Julhttp://www.ncbi.nlm.nih.gov/pubmed/23744338
Feed intake, growth performance and cholesterol metabolism in juvenile turbot (Scophthalmus maximus L.) fed defatted fish meal diets with graded levels of cholesterol Aquaculture 2.009Tengfei Zhu, et al. 2014 Mayhttp://www.sciencedirect.com/science/article/pii/S0044848614001306
Amino acid substitutions of GLY98, LEU245 and GLU543 in COI1 distinctively affect jasmonate-regulated male fertility in Arabidopsis Science China Life Sciences 1.688Huang Huang, et al. 2014 Janhttp://link.springer.com/article/10.1007/s11427-013-4590-1
Protective effect of polysaccharides on simulated microgravity-induced functional inhibition of human NK cells Carbohydrate Polymers 3.479Ting Huyan, et al. 2014 Janhttp://www.sciencedirect.com/science/article/pii/S0144861713010321
A fungal protein elicitor PevD1 induces Verticillium wilt resistance in cotton Plant Cell Reports 2.509Bingwu Bu, et al. 2014 Marhttp://link.springer.com/article/10.1007/s00299-013-1546-7
Modulation of HBV replication by microRNA-15b through targeting hepatocyte nuclear factor 1α Nucleic Acids Research 8.278Xiaopeng Dai, et al. 2014 Aprhttp://nar.oxfordjournals.org/content/early/2014/04/04/nar.gku260.short
Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma Applied Biochemistry and Biotechnology 1.893He Hao, et al. 2014 Junhttp://link.springer.com/article/10.1007/s12010-014-1025-9
Modulation of modeled microgravity on radiation-induced bystandereffects in Arabidopsis thalianaTing Mutation Research/Fundamental and MolecularMechanisms of Mutagenesis 4Ting Wanga, et al. 2015 Janhttp://dx.doi.org/10.1016/j.mrfmmm.2015.01.010
Arabidopsis BREVIPEDICELLUS Interacts with the SWI2/SNF2 Chromatin Remodeling ATPase BRAHMA to Regulate KNAT2 and KNAT6 Expression in Control of Inflorescence Architecture PLOS Genetics 8.167Minglei Zhao, et al. 2015 Marhttp://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005125
Arabidopsis histone demethylases LDL1 and LDL2 control primary seed dormancy by regulating DELAY OF GERMINATION 1 and ABA signaling-related genes  Frontiers in Plant Science 3.637Minglei Zhao, et al. 2015 Marhttp://journal.frontiersin.org/article/10.3389/fpls.2015.00159/abstract
Identification and characterization of histonedeacetylasesintomato(Solanumlycopersicum) Frontiers in Plant Science 3.637Linmao Zhao, et al.  2015 Janhttp://journal.frontiersin.org/article/10.3389/fpls.2014.00760/abstract
Identification of miR-2400 gene as a novel regulator in skeletal muscle satellite cells proliferation by targeting MYOG gene Biochemical and Biophysical Research Communications 2.297Wei Wei Zhang, et al.  2015 Junhttp://www.sciencedirect.com/science/article/pii/S0006291X15300358
Characterization of GST genes from the Chinese white pine beetle Dendroctonus armandi (Curculionidae: Scolytinae) and their response to host chemical defense Pest Management Science 2.694Lulu Dai, et al.    2015 Junhttp://onlinelibrary.wiley.com/doi/10.1002/ps.4059/abstract
Bitter taste receptor mTas2r105 is expressed in small intestinal villus and crypts Biochemical and Biophysical Research Communications 2.297Fu Gu, et al.  2015 Junhttp://www.sciencedirect.com/science/article/pii/S0006291X15300875
Overexpression of TsApx1 from Thellungiella salsuginea improves abiotic stress tolerance in transgenic Arabidopsis thaliana Biologia Plantarum 1.849Z. Q. Li, et al. 2015 Julhttp://link.springer.com/article/10.1007/s10535-015-0533-y
Isolation of a maize ZmCI-1B promoter and characterization
of its activity in transgenic maize and tobacco
Plant cell reports 3.071Ye Li, et al.  2015 Mayhttp://link.springer.com/article/10.1007%2Fs00299-015-1799-4
Overexpression of ZmIRT1 and ZmZIP3 Enhances Iron and Zinc Accumulation in
Transgenic Arabidopsis
PLOS ONE 3.234Suzhen Li, et al. 2015 Aughttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136647
Identification of a novel partial agonist of liver X receptor α (LXRα) via screening Biochemical Pharmacology 4.65Ni Li, et al. 2014 Dechttp://www.ncbi.nlm.nih.gov/pubmed/25450668

 

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TransScript®  One-Step gDNA Removal and  cDNA Synthesis SuperMix

  • Views: 572
  • Product Code: AT311
  • Availability: In Stock
  • $435.00

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