TransTaq® High Fidelity (HiFi) PCR SuperMix I or II is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer. TransTaq® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA and PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.

TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.



• Complex templates
• GC/AT-rich templates
• Long PCR
• High yield PCR



Contents& storage



Citations & references
Literature Journal IF Author Date Link
A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus Virology Journal 2.092 Dai J. et al. 2013 Jan
High prevalence and molecular analysis of macrolide-nonsusceptible Moraxella catarrhalis isolated from nasopharynx of healthy children in China Microbial Drug Resistance 2.364 Liu Y, et al. 2012 Aug
Molecular Adjuvant Ag85A Enhances Protection against Influenza A Virus in Mice Following DNA Vaccination Viruses 2.509 Dai J. et al. 2012 Dec 
Detection of astrovirus infection in pigeons (Columbia livia) during an outbreak of diarrhoea Avian Pathology 1.729 Zhao W, et al. 2011 Aug
Microsatellite markers for the Chinese herbaceous peony Paeonia lactiflora (Paeoniaceae) American Journal of Botany 2.586 Li Lin, et al. 2011 Feb
rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner Cellular & Molecular Immunology 4.112 Jing Hao, et al.  2014 Nov
Proteomic analysis on zoxamide-induced sensitivity changes in Phytophthora cactorum Pesticide Biochemistry and Physiology 2.014 Xinyue Mei, et al. 2015 Jan
ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate Fish & Shellfish Immunology 2.674 Jian Zhang, et al.  2015 May
Molecular cloning and characterization of an Hsp70 gene from the bloom green alga Chaetomorpha valida (Cladophorales, Chlorophyta) Journal of Applied Phycology 2.559 Yunyan Deng, et al. 2014 Apr
Parallel tagged amplicon sequencing of relatively long PCR products using the Illumina HiSeq platform and transcriptome assembly Molecular Ecology Resources 3.712 Yan-Jie Feng, et al.  2015 May;jsessionid=
Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidumssp. dicoccoides ) in relation to Glu -3evolution Development Genes and Evolution 2.441 Lumin Qin, et al.  2014 Nov
Epitope analysis, expression and protection of SAG5A vaccine against Toxoplasma gondii Acta Tropica 2.27 Gang Lu, et al. 2015 Jun
Near Full-Length Identification of a Novel HIV-1 CRF01_AE/B/C Recombinant in Northern Myanmar AIDS Research and Human Retroviruses 2.325 Zhou Yan-Heng, et al. 2015 May
Phytoplasma associated with Chinese tallow tree yellowing disease in China represents a new 16SrIII subgroup Forest Pathology 1.373 Y. Gao, et al.    2014 Jun
Molecular characterizationofanovelhypovirusfromtheplant
pathogenicfungus Fusarium graminearum
Virology 3.321 Pengfei Li, et al. 2015 Jul
Cloning and characterisation of NADPH-dependent cytochrome P450 reductase gene in the cotton bollworm, Helicoverpa armigera Pest Management Science 2.594 Chunqing Zhao, et al. 2014 Jan


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2×TransTaq High Fidelity(HiFi)PCR SuperMix I (-dye) 2×TransTaq High Fidelity(HiFi)PCR SuperMix II (-dye)
Storage: at -20℃ for two years

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2×TransTaq® High Fidelity(HiFi)PCR SuperMix  (-dye )

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  • Product Code: AS131
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2×TransTaq High Fidelity(HiFi)PCR SuperMix I (-dye) 2×TransTaq High Fidelity(HiFi)PCR SuperMix II (-dye)
Storage: at -20℃ for two years
  • $125.00

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