AQ401

Description

TransStart® Probe qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except probe, primer and template. It contains TransStart® Taq DNA Polymerase, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, probe, passive reference dye (optional) and ddH2O.

 

Highlights

TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and
generates more accurate data.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.

 

Applications

N

 

Passive Reference Dye

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

 

 

Contents& storage

 

Storage:
at -20°C in dark for one year
 
 
Citations & references

 

LiteratureJournalIFAuthorDateLink
TaqMan MGB Probe Fluorescence Real-Time Quantitative PCR for Rapid Detection of Chinese Sacbrood Virus PLoS One 3.23Mingxiao M, et al. 2013http://www.ncbi.nlm.nih.gov/pubmed/23408931
Improving the Secretion of a Methyl Parathion Hydrolase in Pichia pastoris by Modifying Its N-Terminal Sequence PLoS One 3.23Ping Wang, et al. 2014 Mayhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.
0096974#pone-0096974-g005

 

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TransStart®  Probe qPCR SuperMix

  • Views: 272
  • Product Code: AQ401
  • Availability: In Stock
  • $155.00

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