TransScript® II Green One-Step qRT-PCR SuperMix combines the firststrand cDNA synthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. TransScript® II Green One-Step qRT-PCR SuperMix contains all the necessary reagents for cDNA synthesis and qPCR except total RNA/mRNA template and gene specific primers.



• 5 minutes cDNA synthesis
• cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
• Passive reference dyes are provided for different qPCR instruments.



• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• Viral RNA and trace RNA detection


Passive Reference Dye

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q



Contents& storage



Citations & references


Transcriptome-wide analysis of TDP-43 binding small RNAs identifies miR-NID1, a novel miRNA that represses NRXN1 expression Genomics 3.01Fan Z, et al. 2013 Jul
A differential sequencing-based analysis of the C. elegans noncoding transcriptome RNA 5.088Xiao T, et al. 2012 Apr
Regulatory elements of Caenorhabditis elegans ribosomal protein genes BMC Genomics 4.397Sleumer, et al. 2012
Functional Characterization of Long Noncoding RNA Lnc_bc060912 in Human Lung Carcinoma Cells Biochemistry  3.015Huaxia Luo, et al. 2015 Apr
The Loss of MiR-139-5p Promotes Colitis-Associated Tumorigenesis by Mediating PI3K/AKT/Wnt Signaling The International Journal of Biochemistry & Cell Biology 4.046Rurong Mao, et al.  2015 Oct
Identification of Intermediate-Size Non-Coding RNAs Involved in the UV-Induced DNA Damage Response in C. elegans PLoS One 3.23Aqian Li, et al. 2012 Nov
Comparative Transcriptome Analysis Reveals Different Molecular Mechanisms of Bacillus coagulans 2-6 Response to Sodium Lactate and Calcium Lactate during Lactic Acid Production PLoS One 3.23Qin J, et al. 2012 Aug


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TransScript®  II  Green One-Step qRT-PCR SuperMix

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  • Product Code: AQ311
  • Availability: In Stock
  • $560.00

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