AQ221

Description

TransScript® Probe One-Step qRT-PCR SuperMix combines the firststrand cDNA synthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. TransScript® Probe One-Step qRT-PCR SuperMix contains all the necessary reagents for cDNA synthesis and qPCR except probe, total RNA/mRNA template and gene specific primers.

 

Highlights

• 5 minutes cDNA synthesis
• cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
• Passive reference dyes are provided for different qPCR instruments.

 

Applications

• Multiple copy and low copy gene detection
• Viral RNA and trace RNA detection

 

Passive Reference Dye

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

 

 

Contents& storage

 

 

Citations & references

 

LiteratureJournalIFAuthorDateLink
The swine CD81 enhances E2-based DNA vaccination against classical swine fever Vaccine 3.624Wenliang Li, et al. 2015 Julhttp://www.sciencedirect.com/science/article/pii/S0264410X15007185

 

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TransScript®   Probe One-Step qRT-PCR SuperMix

  • Views: 251
  • Product Code: AQ221
  • Availability: In Stock
  • $315.00

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