TransScript® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for miRNA detection. High efficient poly(A) tail addition and first-strand cDNA synthesis are performed by TransScript® miRNA RT Enzyme Mix (containing tailing enzyme and RT enzyme) and 2×TS miRNA Reaction Mix. TransScript® Tip Green qPCR SuperMix is provided for miRNA detection.
• One-Step poly(A) tailing and cDNA synthesis.
• Passive reference dyes are provided for different qPCR instruments.
Multiple copy and low copy gene detection
Passive Reference Dye
• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q
at -20°C in dark for one year
TransScript® Green miRNA Two-Step qRT-PCR SuperMix