AQ111

Description

TransStart® Green qPCR SuperMix UDG is a ready-to-use qPCR cocktail containing all components, except primer and template. It contains TransStart® Taq DNA Polymerase, UDG, SYBR Green I, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

 

Highlights

TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.
• UDG and dUTP avoid cross contamination.

 

Applications

N

 

Passive Reference Dye

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

 

 

Contents& storage

 

Storage:
at -20°C in dark for one year

 

Citations & references

 

LiteratureJournalIFAuthorDateLink
Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsiPLoS One3.73Wang B. et al.2013http://www.ncbi.nlm.nih.gov/pubmed/23342036
Identification by cDNA cloning of abundant sRNAs in a human-avirulent Yersinia pestis strain grown under five different growth conditionsFuture Microbiology4.018Qu Y, et al.2012 Aprhttp://www.ncbi.nlm.nih.gov/pubmed/22439729
Tunneling-nanotube direction determination in neurons and astrocytesCell Death and Disease6.044Sun X, et al.2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/23222508
Oral vaccination with attenuated Salmonella enterica serovar Typhimurium expressing Cap protein of PCV2 and its immunogenicity in mouse and swine modelsVeterinary Microbiology3.127Xu XG, et al.2012 Jun http://www.ncbi.nlm.nih.gov/pubmed/22326539
Cellular tropism, population dynamics, host range and taxonomic status of an aphid secondary symbiont, SMLS (Sitobion miscanthi L type symbiont).PLoS One3.23Li T, et al.2011http://www.ncbi.nlm.nih.gov/pubmed/21789197
Selection of reference genes for gene expression studies in ultraviolet B-irradiated human skin fibroblasts using quantitative real-time PCRBMC Molecular Biology2.857Li L. et al.2011 Feb http://www.ncbi.nlm.nih.gov/pubmed/21324211
Morphine protects against intracellular amyloid toxicity by inducing estradiol release and upregulation of Hsp70The Journal of Neuroscience6.908Cui J, et al.2011 Nov http://www.ncbi.nlm.nih.gov/pubmed/22072674
MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1Nucleic Acids Research8.278Liu K, et al.2011 Aprhttp://www.ncbi.nlm.nih.gov/pubmed/21131276
Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica MaximJournal of Integrative Plant Biology2.534Li M, et al.2013 Junhttp://www.ncbi.nlm.nih.gov/pubmed/23480422
Isoflurane Increases Neuronal Cell Death Vulnerability by Downregulating miR-214PLoS One3.23Yan H, et al.2013 Febhttp://www.ncbi.nlm.nih.gov/pubmed/23408966
Molecular characterizationofanovelhypovirusfromtheplant
pathogenicfungus Fusarium graminearum
Virology3.321Pengfei Li, et al.2015 Julhttp://www.sciencedirect.com/science/article/pii/S0042682215001129
TLR2 and TLR4 signaling pathways are required for recombinant Brucella abortus BCSP31-induced cytokine production, functional upregulation of mouse macrophages, and the Th1 immune response in vivo and in vitroCellular & Molecular Immunology3.419Li Jia-Yun,et al.2014 Aprhttp://www.nature.com/cmi/journal/vaop/ncurrent/full/cmi201428a.html

 

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TransStart®  Green qPCR SuperMix UDG

  • Views: 414
  • Product Code: AQ111
  • Availability: In Stock
  • $220.00

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