AQ101

Description

TransStart® Green qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except primer and template. It contains TransStart® Taq DNA Polymerase, SYBR Green I, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

 

Highlights

• TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.

 

Applications

N

 

Passive Reference Dye

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

 

 

Contents& storage

 

 

Citations & references

 

LiteratureJournalIFAuthorDateLink
Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild ricePhytopathology2.968Lv Q, et al.2013 Junhttp://www.ncbi.nlm.nih.gov/pubmed/23384860
VscO, a putative T3SS chaperone escort of Vibrio alginolyticus, contributes to virulence in fish and is a target for vaccine developmentFish & Shellfish Immunology2.964Zhou Z, et al.2013 Novhttp://www.ncbi.nlm.nih.gov/pubmed/23994282
Proteomic comparison of the probiotic bacterium Lactobacillus casei Zhang cultivated in milk and soy milkJournal of Dairy Science2.566Wang J, et al.2013 Sephttp://www.ncbi.nlm.nih.gov/pubmed/23871367
12-Deoxyphorbol 13-palmitate mediated cell growth inhibition, G2-M cell cycle arrest and apoptosis in BGC823 cellsEuropean Journal of Pharmacology2.592Xu HY, et al.2013 Jan http://www.ncbi.nlm.nih.gov/pubmed/23220710
Expression and immunogenicity analysis of accessory colonization factor A from Vibrio alginolyticus strain HY9901Fish & Shellfish Immunology2.964Cai SH, et al.2013 Febhttp://www.ncbi.nlm.nih.gov/pubmed/23261505
Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsiPLoS One3.23Wang B. et al.2013http://www.ncbi.nlm.nih.gov/pubmed/23342036
An S-domain receptor-like kinase OsSIK2 confers abiotic stress tolerance and delays dark-induced leaf senescence in ricePlant Physiology6.555Chen LJ, et al.2013 Oct http://www.ncbi.nlm.nih.gov/pubmed/24143807
Silencing of periostin inhibits nicotine-mediated tumor cell growth and epithelial-mesenchymal transition in lung cancer cellsMolecular Medicine Reports1.17Wu SQ, et al.2013 Marhttp://www.ncbi.nlm.nih.gov/pubmed/23314871
Decreased expression of SOX6 confers a poor prognosis in hepatocellular carcinomaCancer Epidermiology2.232Guo X, et al.2013 Octhttp://www.ncbi.nlm.nih.gov/pubmed/23731550
Cloning and characterization of the dopamine like receptor in the oyster Crassostrea angulata: expression during the ovarian cycleComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology2.069Yang B, et al.2013 Marhttp://www.ncbi.nlm.nih.gov/pubmed/23274282
Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seedsBiochemistry (Moscow)1.149Omar SA, et al.2013 Mayhttp://www.ncbi.nlm.nih.gov/pubmed/238481510
Tunneling-nanotube direction determination in neurons and astrocytesCell Death and Disease6.044Sun X, et al.2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/23222508
Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarctionPLoS One3.23Long G, et al.2012http://www.ncbi.nlm.nih.gov/pubmed/23236408
Silencing of a novel tumor metastasis suppressor gene LASS2/TMSG1 promotes invasion of prostate cancer cell in vitro through increase of vacuolar ATPase activityJournal of Cellular Biochemistry3.062Xu X, et al. 2012 Julhttp://www.ncbi.nlm.nih.gov/pubmed/22573553
Identification by cDNA cloning of abundant sRNAs in a human-avirulent Yersinia pestis strain grown under five different growth conditionsFuture Microbiology4.018Qu Y, et al.2012 Aprhttp://www.ncbi.nlm.nih.gov/pubmed/22439729
Cloning and expression analysis of recombination activating genes (RAG1/2) in red snapper (Lutjanus sanguineus). Fish & Shellfish Immunology2.964Zhang XL, et al.2012 Aprhttp://www.ncbi.nlm.nih.gov/pubmed/22266137
The therapeutic effects of daphnetin in collagen-induced arthritis involve its regulation of Th17 cellsInternational Immunopharmacology2.417Tu L. et al.2012 Aughttp://www.ncbi.nlm.nih.gov/pubmed/22554834
Effects of low temperatures on proliferation-related signaling pathways in the hippocampus after traumatic brain injuryExperimental Biology and Medicine2.803Wang L, et al.2012 Dechttp://www.ncbi.nlm.nih.gov/pubmed/23354401
The tumor suppressor role of Src homology phosphotyrosine phosphatase 2 in hepatocellular carcinomaJournal of Cancer Research and Clinical Oncology2.914Jiang C, et al.2012 Aprhttp://www.ncbi.nlm.nih.gov/pubmed/22228034
Pyrosequencing of Haliotis diversicolor transcriptomes: insights into early developmental molluscan gene expressionPLoS One3.73Huang ZX, et al.2012http://www.ncbi.nlm.nih.gov/pubmed/23236463
Stable expression of Y-box protein 1 gene in early development of the abalone Haliotis diversicolorThe International Journal of Developmental Biology2.614Chen J, et al.2012http://www.ncbi.nlm.nih.gov/pubmed/22689369
SOX2 gene regulates the transcriptional network of oncogenes and affects tumorigenesis of human lung cancer cellsPLoS One3.23Chen S, et al.2012http://www.ncbi.nlm.nih.gov/pubmed/22615765
Oral vaccination with attenuated Salmonella enterica serovar Typhimurium expressing Cap protein of PCV2 and its immunogenicity in mouse and swine modelsVeterinary Microbiology3.127Xu XG, et al.2012 Jun http://www.ncbi.nlm.nih.gov/pubmed/22326539
Overexpression of lysine specific demethylase 1 predicts worse prognosis in primary hepatocellular carcinoma patientsWorld Journal of Gastroenterology2.547Zhao ZK, et al.2012 Dechttp://www.ncbi.nlm.nih.gov/pubmed/23236241
Effect of hypaconitine combined with liquiritin on the expression of calmodulin and connexin43 in rat cardiac muscle in vivoJournal of Pharmacy and Pharmacology2.033Yi M, et al.2012 Novhttp://www.ncbi.nlm.nih.gov/pubmed/23058053
Effects of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat dental pulp stem cellsMolecular Medicine Reports1.17Huang XF, et al.2012 Sephttp://www.ncbi.nlm.nih.gov/pubmed/22751806
Nucleofection of a DNA vaccine into human monocyte-derived dendritic cellsCellular Immunology1.743Xie Q, et al.2012 Marhttp://www.ncbi.nlm.nih.gov/pubmed/22632899
Side-effects of resveratrol in HepG2 cells: Reduced pten and increased bcl-xl mRNA expressionMolecular Medicine Reports1.17Zheng M, et al.2012 Dechttp://www.ncbi.nlm.nih.gov/pubmed/22971900
 Expression features of SOX9 associate with tumor progression and poor prognosis of hepatocellular carcinomaDiagn Pathol1.85Guo X, et al. 2012 Aprhttp://www.ncbi.nlm.nih.gov/pubmed/22515642
Molecular characterization and functional analysis of adrenergic like receptor during larval metamorphosis in Crassostrea angulataAquaculture2.009Yang B, et al.2012 Novhttp://www.sciencedirect.com/science/article/pii/S0044848612005212
PKHD1 post‐transcriptionally modulated by miR‐365‐1 inhibits cell‐cell adhesionCell Biochemistry and Function1.854Duan J, et al.2012 Julhttp://www.ncbi.nlm.nih.gov/pubmed/22411058
Cellular tropism, population dynamics, host range and taxonomic status of an aphid secondary symbiont, SMLS (Sitobion miscanthi L type symbiont). PLoS One3.23Li T, et al.2011http://www.ncbi.nlm.nih.gov/pubmed/21789197
MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1Nucleic Acids Research8.278Liu K, et al.2011 Aprhttp://www.ncbi.nlm.nih.gov/pubmed/21131276
Morphine protects against intracellular amyloid toxicity by inducing estradiol release and upregulation of Hsp70The Journal of Neuroscience6.908Cui J, et al.2011 Nov http://www.ncbi.nlm.nih.gov/pubmed/22072674
Selection of reference genes for gene expression studies in ultraviolet B-irradiated human skin fibroblasts using quantitative real-time PCRBMC Molecular Biology2.857Li L. et al.2011 Feb http://www.ncbi.nlm.nih.gov/pubmed/21324211
Identification and expression analysis of two small heat shock protein cDNAs from developing seeds of biodiesel feedstock plant Jatropha curcasPlant Science 2.922Omar SA, et al.2011 Dechttp://www.ncbi.nlm.nih.gov/pubmed/21958704
NIMA-related kinase NEK6 affects plant growth and stress response in ArabidopsisThe Plant Jounal6.16Zhang B, et al.2011 Dechttp://www.ncbi.nlm.nih.gov/pubmed/21801253
Effect of strychnine hydrochloride on liver cytochrome P450 mRNA expression and monooxygenase activities in ratActa Pharmaceutica Sinica B2.354Gao Q, et al.2011 Aughttp://www.sciencedirect.com/science/article/pii/S2211383511000475
PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosteroneThe Biochemical Journal 4.654Zhu L, et al.2010 Dec http://www.ncbi.nlm.nih.gov/pubmed/21108604
MicroRNA-126 modulates the tumor microenvironment by targeting calmodulin-regulated spectrin-associated protein 1 (Camsap1)International Journal of Oncology2.657Xin Sun, et al.2014 Mayhttp://www.spandidos-publications.com/ijo/44/5/1678?text=fulltext
Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenic Fusarium oxysporum CS-20FEMS Microbiology Letters2.049Xiaoming Pu, et al.2014 Mayhttp://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12461/abstract;jsessionid=
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TransStart®  Green qPCR SuperMix

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  • Product Code: AQ101
  • Availability: In Stock
  • $155.00

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