AP221

Description

TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. TransStart® FastPfu Fly DNA Polymerase has an xtension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.

 

Highlights

TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-6 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

 

Applications

• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates

 

Unit Definition

One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

TransStart® FastPfu Fly DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu Fly DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

 

Storage Buffer

50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol

 

TransStart® FastPfu Fly Buffer

100 mM Tris-SO4 pH 9.2, 50 mM (NH4)2SO4, 200 mM KCl; 10 mM MgSO4; 10% Glycerol; others

 

Contents& storage

Citations & references
Literature Journal IF Author Date Link
The GADD45A (1506T>C) Polymorphism Is Associated with Ovarian Cancer Susceptibility and Prognosis Plos one 3.234 Cunzhong Yuan, et al. 2015 Sep  http://www.plosone.org/article/Authors/info:doi/10.1371
/journal.pone.0138692

 

Related Images

TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. The fidelity of TransStart® FastPfu Fly DNA Polymerase is 108 times higher than EasyTaq® DNA polymerase. The extension rate up to 6 kb/min.
Storage: at -20 ℃ for two years

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TransStart®  FastPfu DNA Polymerase

  • Views: 632
  • Product Code: AP221
  • Availability: In Stock
TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. The fidelity of TransStart® FastPfu Fly DNA Polymerase is 108 times higher than EasyTaq® DNA polymerase. The extension rate up to 6 kb/min.
Storage: at -20 ℃ for two years
  • $240.00

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