Ribonuclease Inhibitor is a recombinant protein purified from E. coli strain carrying human placenta ribonuclease inhibitor gene. Ribonuclease Inhibitor specifically inhibits RNase A, RNase B, and RNase C. It is not effective against RNase 1, RNase T1, S1 nuclease, RNase H and aspergillus-originated RNase. It has no inhibition effect on DNA Polymerase, AMV, M-MLV, SP6, T7 and T3 RNA Polymerases.
50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 10 mM DTT, 100 mM NaCl, 50% glycerol
One unit is defined as the amount of enzyme required to inhibit 5 ng RNase A by 50%.
In vitro inhibition of ribonuclease, cDNA synthesis and in vitro transcription and translation.
1. Reaction Components
• For anchored oligo(dT)18 primer or GSP, incubate at 42℃ for 30 minutes.
• For random primer, incubate at 25℃ for 10 minutes, then at 42℃ for 30 minutes.
3. Incubate at 85℃ for 5 seconds to inactivate EasyScript®/TransScript® RT.