AH411

Description

One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. TransScript® II RT and TransTaq® HiFi DNA Polymerase are used in the kit.

 

Highlights

Amplification of fragment up to 8 kb.

 

Applications

• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

 

 

Contents& storage

 

 

Citations & references

 

LiteratureJournalIFAuthorDateLink
Transcriptome-wide analysis of TDP-43 binding small RNAs identifies miR-NID1, a novel miRNA that represses NRXN1 expression Genomics 3.01Fan Z, et al. 2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/23827811
A differential sequencing-based analysis of the C. elegans noncoding transcriptome RNA 5.088Xiao T, et al. 2012 Aprhttp://www.ncbi.nlm.nih.gov/pubmed/22345127

 

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TransScript®  II  One-Step RT-PCR SuperMix (+dye)

  • Views: 323
  • Product Code: AH411
  • Availability: In Stock
  • $1,315.00

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