AH341

Description

The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.

 

Highlights

• Simultaneous genomic DNA removal and cDNA synthesis.
• The optimal ratio of Oligo(dT)20 Primer to random primer(N9) for qPCR ready cDNA.
• qPCR ready cDNA in 15 minutes.
• cDNA up to 250 bp.

 

Applications

• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

 

 

Contents& storage

 

 

Citations & references

 

LiteratureJournalIFAuthorDateLink
Clinicopathological significance of orphan nuclear receptor Nurr1 expression in gastric cancer Clinical and Translational Oncology 2.077J. Guo, et al.   2015 Mayhttp://link.springer.com/article/10.1007/s12094-015-1305-z
Exploring the formation and recognition of an important G-quadruplex in a HIF1α promoter and its transcriptional inhibition by a benzo [c] phenanthridine derivative journal of the american chemical society 11.444Han Chen, et al. 2014http://pubs.acs.org/doi/abs/10.1021/ja412128w

 

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TransScript®  II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )

  • Views: 230
  • Product Code: AH341
  • Availability: In Stock
  • $910.00

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