AH301

Description

TransScript® II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript® II RT/RI Enzyme Mix and 2×TS II Reaction Mix.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
• cDNA up to 15 kb.

 

Highlights

N

 

Applications

• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

 

 

Contents& storage

 

 

Citations & references

 

LiteratureJournalIFAuthorDateLink
The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice PLoS One 3.23Zhang Y, et al. 2013http://www.ncbi.nlm.nih.gov/pubmed/23544067
Nspc1 regulates the key pluripotent Oct4-Nanog-Sox2 axis in P19 embryonal carcinoma cells via directly activating Oct4 Biochemical and Biophysical Research Communications 2.406Li H, et al. 2013 Nov http://www.ncbi.nlm.nih.gov/pubmed/24113379
Desflurane accelerates neuronal cytotoxicity of Aβ by downregulating miR-214 Neuroscience Letters 2.026Yu Y, Zhang Y. 2013 Sep http://www.ncbi.nlm.nih.gov/pubmed/24021802
Saturated fatty acid palmitate-induced insulin resistance is accompanied with myotube loss and the impaired expression of health benefit myokine genes in C2C12 myotubes Lipids in Health and Disease  2.015Yang M, et al. 2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/23866690
Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi PLoS One 3.23Wang B. et al. 2013http://www.ncbi.nlm.nih.gov/pubmed/23342036
Isoflurane Increases Neuronal Cell Death Vulnerability by Downregulating miR-214 PLoS One 3.23Yan H, et al. 2013http://www.ncbi.nlm.nih.gov/pubmed/23408966
Nitric Oxide Mediates Cytokinin Functions in Cell Proliferation and Meristem Maintenance in Arabidopsis Molecular Plant 6.126Shen Q, et al. 2013 Julhttp://www.ncbi.nlm.nih.gov/pubmed/23239827
An improved CTAB-ammonium acetate method for total RNA isolation from cotton Phytochemical Analysis 2.48Zhao L, et al. 2012 Novhttp://www.ncbi.nlm.nih.gov/pubmed/22552877
Tunneling-nanotube direction determination in neurons and astrocytes Cell Death and Disease 6.044Sun X, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/23222508
GABA attenuates amyloid toxicity by downregulating its endocytosis and improves cognitive impairment Journal of Alzheimer's Disease 4.174Sun X, et al. 2012http://www.ncbi.nlm.nih.gov/pubmed/22672879
Role of nuclear receptor coactivator 3 (ncoa3) in pluripotency maintenance Journal of Biological Chemistry 4.773Wu Z, et al. 2012 Novhttp://www.ncbi.nlm.nih.gov/pubmed/22977234
Murine myeloid dendritic cells that phagocytose apoptotic T cells inhibit the immune response via NO PLoS One 3.23Zhong K, et al. 2012http://www.ncbi.nlm.nih.gov/pubmed/23166651
Tunneling-nanotube development in astrocytes depends on p53 activation Cell Death and Differentiation 8.371Wang Y, et al. 2011 Aprhttp://www.ncbi.nlm.nih.gov/pubmed/21113142
Obligate mutualism within a host drives the extreme specialization of a fig wasp genome Genome Biology 10.288Jin-Hua Xiao,et al. 2013 Dechttp://genomebiology.com/2013/14/12/R141
Morphine protects against intracellular amyloid toxicity by inducing estradiol release and upregulation of Hsp70 The Journal of Neuroscience 6.908Cui J, et al. 2011 Nov http://www.ncbi.nlm.nih.gov/pubmed/22072674
Growth and stress responses of the earthworm Eisenia fetida to Escherichia coli O157:H7 in an artificial soil Microbial Pathogenesis 1.974Liu X, et al. 2009 Mayhttp://www.ncbi.nlm.nih.gov/pubmed/19232388
The Batten disease gene CLN3 confers resistance to endoplasmic reticulum stress induced by tunicamycin Biochemical and Biophysical Research Communications 2.406Dan Wu, et al. 2014 Aprhttp://www.sciencedirect.com/science/article/pii/S0006291X14005725
Adaptive evolution of the circadian gene timeout in insects Scientific Reports 2.927Haifeng Gu, et al. 2014 Febhttp://www.nature.com/srep/2014/140227/srep04212/full/srep04212.html?
WT.ec_id=SREP-631-20140304#affil-auth

 

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TransScript®  II First-Strand cDNA Synthesis SuperMix

  • Views: 417
  • Product Code: AH301
  • Availability: In Stock
  • $760.00

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