AE301

Description

EasyScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is ef ficiently synthesized by EasyScript® RT/RI Enzyme Mix and 2×ES Reaction Mix.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the, product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 8 kb.

 

Highlights

N

 

Applications

Multiple copy gene detection

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Decreased fructose-1, 6-bisphosphatase-2 expression promotes glycolysis and growth in gastric cancer cells Molecular Cancer 5.134 Li H, et al. 2013 http://www.ncbi.nlm.nih.gov/pubmed/24063558
miR-375 targets the p53 gene to regulate cellular response to ionizing radiation and etoposide in gastric cancer cells DNA Repair 4.274 Liu Y, et al. 2013 Sep http://www.ncbi.nlm.nih.gov/pubmed/23835407
Cotton GhMPK6a negatively regulates osmotic tolerance and bacterial infection in transgenic Nicotiana benthamiana, and plays a pivotal role in development The FEBS Journal 4.25 Li Y, et al.  2013 Oct http://www.ncbi.nlm.nih.gov/pubmed/23957843
Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo Food and Chemical Toxicology 3.01 Wang X, et al. 2013 Oct http://www.ncbi.nlm.nih.gov/pubmed/23891578
A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress Cell Stress and Chaperones 2.484 Zhang Y, et al. 2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/23382010
MiR-18b inhibites TGF-ß1-induced differentiation of hair follicle stem cells into smooth muscle cells by targeting SMAD2 Biochemical and Biophysical Research Communications 2.406 Liu X, et al. 2013 Aug  http://www.ncbi.nlm.nih.gov/pubmed/23916701
Identification, genomic organization, and oxidative stress response of a sigma class glutathione S-transferase gene (AccGSTS1) in the honey bee, Apis cerana cerana Cell Stress and Chaperones 2.484 Yan H, et al. 2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/23250585
A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus Virology Journal 2.092 Dai J. et al. 2013 Jan http://www.ncbi.nlm.nih.gov/pubmed/23369570
Effects of Aqueous Extracts of Raw Pu‐erh Tea and Ripened Pu‐Erh Tea on Proliferation and Differentiation of 3T3‐L1 Preadipocytes Phytotherapy Research 2.068 Cao ZH, et al. 2013 Aug http://www.ncbi.nlm.nih.gov/pubmed/23027678
12-Deoxyphorbol 13-palmitate mediated cell growth inhibition, G2-M cell cycle arrest and apoptosis in BGC823 cells European Journal of Pharmacology 2.592 Xu HY, et al. 2013 Jan  http://www.ncbi.nlm.nih.gov/pubmed/23220710
Expression of HGF and Met in Human Tissues of Colorectal Cancers: Biological and Clinical Implications for Synchronous Liver Metastasis International Journal of Medical Sciences 2.065 Sun YL, et al. 2013 http://www.ncbi.nlm.nih.gov/pubmed/23532910
Compensation phenomena found in Acidithiobacillus ferrooxidans after starvation stress Journal of Basic Microbiology 1.198 Tu B, et al. 2013 May http://www.ncbi.nlm.nih.gov/pubmed/23686751
MiR-143 is not essential for adipose development as revealed by in vivo antisense targeting Biotechnology Letters 1.853 He Z, et al. 2013 Apr http://link.springer.com/article/10.1007/s10529-012-1112-3
Oxymatrine ameliorates non-alcoholic fatty liver disease in rats through peroxisome proliferator-activated receptor-α activation Molecular Medicine Reports 1.17 Shi L, et al. 2013 Aug http://www.ncbi.nlm.nih.gov/pubmed/23754536
Notch 1 Signalling Inhibits Cardiomyocyte Apoptosis in Ischaemic Postconditioning Heart, Lung and Circulation 1.254 Yu B, Song B. 2013 Aug  http://www.ncbi.nlm.nih.gov/pubmed/23948289
Monobutyl phthalate induces the expression change of G-Protein-Coupled Receptor 30 in rat testicular Sertoli cells Folia Histochemica et Cytobiologica 1.101 Hu Y, et al. 2013 http://www.ncbi.nlm.nih.gov/pubmed/23690213
Construction and analysis of a subtractive cDNA library of early embryonic development in duck Genetics and Molecular Research 0.994 Liu YL, et al. 2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/23884767
Microcystin (-LR) affects hormones level of male mice by damaging hypothalamic-pituitary system Toxicon 2.924 Wang X, et al. 2012 Feb http://www.ncbi.nlm.nih.gov/pubmed/22178782
The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats Molecular Biology Reports 2.506 Wu L, et al. 2012 Sep http://www.ncbi.nlm.nih.gov/pubmed/22733486
Comparative evaluation of nonylphenol isomers on steroidogenesis of rat Leydig Cells Toxicology In Vitro 2.65 Ying F, et al. 2012 Oct http://www.ncbi.nlm.nih.gov/pubmed/22771390
Inhibition on hepatitis B virus in vitro of lectin from Musca domestica pupa via the activation of NF-κB Virus Research 2.745 Cao X, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/22940568
ER stress negatively modulates the expression of the miR-199a/214 cluster to regulates tumor survival and progression in human hepatocellular cancer PLoS One 3.23 Duan Q, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/22359598
Molecular characterization of a Nicotiana tabacum NtRDR6 gene Plant Molecular Biology Reporter 5.319 Li S, et al. 2012 Dec http://link.springer.com/article/10.1007/s11105-012-0455-1
Cloning and Expression Analysis of Wheat Pheophorbide a Oxygenase Gene TaPaO Plant Molecular Biology Reporter 5.319 Ma N, et al. 2012 Oct http://link.springer.com/article/10.1007/s11105-012-0443-5
Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs Evidence-based Complementary and Alternative Medicine: eCAM 4.774 Wu F, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/23346217
Expression of MMP-9 and WAVE3 in colorectal cancer and its relationship to clinicopathological features Journal of Cancer Research and Clinical Oncology 2.914 Zhang Y, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/22806308
Homoplantaginin Modulates Insulin Sensitivity in Endothelial Cells by Inhibiting Inflammation Biological and Pharmaceutical Bulletin 1.849 Wu F, et al.  2012 http://www.ncbi.nlm.nih.gov/pubmed/22791168
Musca domestica pupae lectin improves the immunomodulatory activity of macrophages by activating nuclear factor-κB Journal of Medicinal Food 1.642 Cao X, et al. 2012 Feb http://www.ncbi.nlm.nih.gov/pubmed/22191632
Construction of the mutant strain in Aspergillus oryzae 3.042 for abundant proteinase production by the N+ ion implantation mutagenesis International Journal of Food Science & Technology 1.24 Zhao G. et al. 2012 Mar  http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.2011.02870.x/abstract?deniedAccessCustomisedMessage=&userIsAuthenticated=false
The expression of pectin methylesterase in onion flower buds is associated with the dominant male-fertility restoration allele Plant Breeding 1.175 Huo Y, et al.  2012 Feb http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2011.01907.x/abstract?deniedAccessCustomisedMessage=&userIsAuthenticated=false
Molecular cloning and characterization of a thioredoxin peroxidase gene from Apis cerana cerana Insect Molecular Biology 2.529 Yu F, et al. 2011 Jun http://www.ncbi.nlm.nih.gov/pubmed/21382109
Lectin purified from Musca domestica pupa up-regulates NO and iNOS production via TLR4/NF-κB signaling pathway in macrophages International Immunopharmacology 2.417 Cao X, et al. 2011 Apr http://www.ncbi.nlm.nih.gov/pubmed/21168541
Generation of pancreatic insulin-producing cells from rhesus monkey induced pluripotent stem cells Diabetologia 6.487 Zhu FF, et al. 2011 Sep http://www.ncbi.nlm.nih.gov/pubmed/21755313
Protection of red snapper (Lutjanus sanguineus) against Vibrio alginolyticus with a DNA vaccine containing flagellin flaA gene Letters in Applied Microbiology 1.629   2011 Feb http://www.ncbi.nlm.nih.gov/pubmed/21198695
Ectodomain shedding of Fcalpha receptor is mediated by ADAM10 and ADAM17 Immunology 2.814 Peng M, et al. 2010 May http://www.ncbi.nlm.nih.gov/pubmed/20059578
The toxic effects of microcystin-LR on rat spermatogonia in vitro Toxicology Letters 3.145 Yuan Zhou, et al. 2012 Jul http://www.sciencedirect.com/science/article/pii/S0378427412011150
Characterization and Mutational Analysis of Omega-Class GST (GSTO1) from Apis cerana cerana, a Gene Involved in Response to Oxidative Stress PLoS One 3.23 Fei Meng, et al. 2014 Mar http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.
0093100;jsessionid=A57E8BED6C41E2476640ED4A6644412B
miR-21 promotes the differentiation of hair follicle-derived neural crest stem cells into Schwann cells Neural Regeneration Research 0.144 Yuxin Ni, et al. 2014 May http://www.nrronline.org/article.asp?issn=1673-5374;year=2014;volume=9;issue=8;spage=828;epage=836;aulast=Ni
Identification and characterisation of a novel 1-Cys thioredoxin peroxidase gene (AccTpx5) from Apis cerana cerana COMP BIOCHEM PHYS B  1.551 Yan Yan, et al. 2014 Jun-Jul http://www.sciencedirect.com/science/article/pii/S1096495914000438
Notch 1 Signalling Inhibits Cardiomyocyte Apoptosis in Ischaemic Postconditioning Heart, Lung and Circulation 1.254 Bentong Yu, et al. 2014 Feb http://www.sciencedirect.com/science/article/pii/S1443950613010809
Swimming improves high-fat induced insulin resistance by regulating lipid and energy metabolism and the insulin pathway in rats International Journal of Molecular Medicine 1.957 Song, et al. 2014 http://www.ingentaconnect.com/content/sp/ijmm/2014/00000033/00000006/art00037

EasyScript First-Strand cDNA Synthesis SuperMix/reverse transcription mastermix
Storage: at -20℃ for two years

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EasyScript® First-Strand cDNA Synthesis SuperMix

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  • Product Code: AE301
  • Availability: In Stock
EasyScript First-Strand cDNA Synthesis SuperMix/reverse transcription mastermix
Storage: at -20℃ for two years
  • $265.00

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